Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic ProbeAditya Kashyap 1, Julien F. Cors 1, Robert D. Lovchik 1, Govind V. Kaigala 1
1IBM Research - Zurich
We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.
